Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil.

نویسندگان

  • Bruna Aparecida Souza Machado
  • Rejane Pina Dantas Silva
  • Gabriele de Abreu Barreto
  • Samantha Serra Costa
  • Danielle Figuerêdo da Silva
  • Hugo Neves Brandão
  • José Luiz Carneiro da Rocha
  • Odir Antônio Dellagostin
  • João Antônio Pegas Henriques
  • Marcelo Andres Umsza-Guez
  • Francine Ferreira Padilha
چکیده

The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection.

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عنوان ژورنال:
  • PloS one

دوره 11 1  شماره 

صفحات  -

تاریخ انتشار 2016